Quality control for assay development
To be confident in your results you need robust and repeatable data. This is where Malvern Panalytical can help you in a number of ways, starting with understanding the quality and stability of assay components such as reagents, proteins or compounds. Reagent control is the basis for good assay development, and reagent stability, purity and functionality must be considered to achieve a robust assay.
Watch the short videos, in the related content below, to see how our solutions can help with the following:
Make sure your target protein is in good condition
When screening many compounds; for example, in a biosensor assay, it is critically important that the target protein is active and stable under assay conditions. An unstable or inactive target protein could compromise the assay, resulting in costly screening iterations and potentially even false negative results.
Address solubility issues with low molecular weight ligands
Solubility issues for low molecular weight (LMW) ligands can also create quality issues in screening activities. Problems with solubility can affect binding data and make your ligand-ranking less reliable.
Ensure all batches of target proteins are the same
Find out how Isothermic Titration Calorimetry is used in quality control of different target protein batches.
Assay development - related content
Enzymes play a special role by catalyzing chemical reactions in the human body through binding to molecular substrates and modifying them in specific ways. Enzymes are important in drug discovery and development, as approximately half of current drug targets are enzymes. A lot of effort is put into discovery and characterization of enzymatic pathways and enzyme activity as well as developing drugs that interact with enzymes
Enzymatic assays are among the most frequently performed activities in biochemistry and typically require use of labeled substrates and coupled reactions with spectrophotometric or chemical readout. Isothermal titration calorimetry (ITC) offers a direct and generic way of following the rate of enzymatic catalysis through the heat rate associated with enzymatic reactions. Enzymatic assays in ITC can be run with opaque solutions at enzyme concentrations comparable to that used in biochemical assays and can yield a complete set of kinetic parameters in a single experiment.
Enzymatic Assays - Featured Content
Validation of hits from primary screens
The hits generated by the high- and medium-throughput screening assays used in the early stages of drug discovery need validation to ensure they aren’t false positives; for example, if they interact with a biochemical assay instead of the target protein. Isothermal Titration Calorimetry (ITC) can be used to confirm and quantify binding and to establish binding stoichiometries, so that false positives and non-stoichiometric binders can be discounted and not waste resource as the project progresses.