OMNISEC

In size-exclusion chromatography, molecules are separated according to their hydrodynamic radius as they enter and exit the pores of a porous gel packing matrix in the column. Like other HPLC-based techniques, this involves the use of a pump, column and at least one detector to measure the sample as it elutes. An OMNISEC GPC/SEC system includes the following components:

Degasser

• Rapid mobile-phase exchange thanks to low volume degasser

Pump

• Rapid mobile-phase exchange thanks to self-priming pump
• Reduced service requirement thanks to novel continuous back seal flushing mechanism

Autosampler

• Protect samples from aggregation with autosampler temperature control
• Use your preferred sample format; vials or 96-well microtiter plates
• Reduce sample use with zero-waste mode

Column Oven

• Temperature control of up to 6 analytical columns

Refractive Index

• Measure the concentration of any solute with excellent sensitivity

UV/Vis PDA

• Measure absorbance at any wavelength from 190-900 nm
• Assess changes in absorbance spectra
• Characterize copolymers

Light Scattering

• Measure absolute molecular weight in a wider range of applications
• Measure as little as 100 ng of material
• Measure molecular weights down to 200 Da

Viscometer

• Better intrinsic viscosity measurements thanks to automated balancing
• Reduced service cost and time thanks to user-exchangeable capillaries

OMNISEC RESOLVE

Pump flow rate:

0.005 - 10 mL/min

Flow rate accuracy:

±1%

Pressure range:

0 - 5000 PSI (34.5 MPa)

Pulsation:

0.15% @ 1 mL/min in water

Dimensions (W, D, H):

420, 640, 890 mm

Weight:

62 kg

Software:

OMNISEC software v10 (or later)

Data acquisition rate:

100 Hz

Patents:

US 14/599,033 "Continuous back seal wash"

AUTOSAMPLER

Capacity:

Up to 192

Sample container types:

HPLC vials or 96-well microtiter plates

Injection volume:

1 - 300 µL

Injection volume accuracy:

>99.5%

Injection volume precision:

<0.3% RSD in full loop mode

<0.5% RSD in partial loop mode

<1% RSD in µL pickup mode

Injection overhead volume:

0 µL in µL pickup mode

Syringe volume:

250 µL standard

Temperature control range:

4 - 60 °C

COLUMN OVEN

Column capacity:

6 x analytical (1 x Tricorn 10/300 GL)

Temperature control range:

20 - 65 °C

OMNISEC REVEAL

Dimensions (W, D, H):

420, 640, 600 mm

Weight:

40 kg

Detector temperature range:

20 - 65 °C

Software:

OMNISEC software v10 (or later)

Data acquisition rate:

100 Hz

Patents:

US20140060162A1 & EP2619543Ba "Modular capillary bridge viscometer"

US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"

Detector 1

Detector:

Light scattering

Measurement principle:

RALS 90° angle, LALS 7° angle

Light source:

50 mW, 640 nm laser

Cell volume:

18 µL

Dynamic range:

2500 mV

Baseline noise:

<0.1 mV

Baseline drift:

<0.2 mV/hr

Detector 2

Detector:

Differential refractive index

Measurement principle:

Deflection

Cell volume:

12 µL

Dynamic range:

±2.5 x 10^-4 RIU

Baseline noise:

<10^-7 RIU

Baseline drift:

<3 x 10^-7 RIU/hr

Detector 3

Detector:

Viscometer

Measurement principle:

4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries

Transducers:

Firmware-based transducer overpressure protection

Detector volume:

17 µL/capillary

Differential pressure baseline noise:

0.3 Pa

Inlet pressure dynamic range:

100 kPa

Inlet pressure baseline noise:

0.01 kPa

Baseline drift:

<0.2 kPa/hr

Detector 4

Detector:

Diode-array-based UV/Vis spectrometer

Wavelength:

190 - 900 nm

Number of wavelengths:

1024

Wavelength accuracy:

<1 nm

Wavelength resolution:

0.6 nm

Cell volume:

7.5 µL

Path length:

10 mm

Baseline noise:

2 x 10^-5 AU

Baseline drift:

5 x 10^-4 AU/hr

Measurement type 1

Measurement type:

Absolute molecular weight

Measurement range:

200 - >10⁷ g/mol

Minimum quantifiable mass:

100 ng of 100kDa molecular weight polystyrene in THF

Measurement principle:

Light scattering (LALS or RALS)

Measurement type 2

Measurement type:

Intrinsic viscosity

Minimum quantifiable mass:

1 µg of 100kDa molecular weight polystyrene in THF

Measurement principle:

4-capillary Wheatstone bridge

Measurement type 3

Measurement type:

Concentration

Minimum quantifiable mass:

100 ng of 100kDa molecular weight polystyrene in THF

Measurement principle:

Differential refractive index detection

Operating environment

Temperature:

15 - 30 °C

Software

Minimum computer specification:

Windows 7 Professional 64 bit, 4th Gen Intel(R) Core I7 Processor (Quad Core HT, 3.4 GHz Turbo, w/ HD Graphics), 8 GB 1600 MHz DDR3 memory, 500 GB 3.5 inch SATA (7200 RPM) HDD, Full-HD monitor

OMNISEC

OMNISEC RESOLVE

Combined pump, degasser, autosampler and column oven, for high quality GPC/SEC and accurate molecular weight measurement.

OMNISEC RESOLVE combines with OMNISEC REVEAL to form a complete OMNISEC GPC/SEC solution for the measurement of absolute molecular weight, intrinsic viscosity and other parameters. It combines an integrated pump, degasser, autosampler and column oven in a single advanced separations unit.

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OMNISEC REVEAL

The ultimate in integrated advanced detection for GPC/SEC and UPLC/UHPLC

Molecular weight, intrinsic viscosity and numerous other parameters can be measured with OMNISEC REVEAL, the world’s most advanced integrated GPC/SEC multi-detector available. It can be added to any existing conventional GPC/SEC system or used in conjunction with OMNISEC RESOLVE to form a complete GPC/SEC solution, to offer comprehensive molecular characterization of synthetic and natural polymers, as well as proteins. The system is now also compatible with UPLC/UHPLC systems such as Waters ACQUITY APC™ for faster and more detailed polymer analysis.

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OMNISEC software

Powerful, agile, easy to use GPC/SEC software for OMNISEC instrument control, data acquisition, analysis and reporting

OMNISEC software is an integrated solution for the advanced analysis of proteins and polymers by GPC/SEC, and is specifically designed for control of OMNISEC RESOLVE and OMNISEC REVEAL. Simplified workflows improve productivity and help you to maximize the information and efficiency of your experiments. Simply load your samples and the software controls all the sequencing, enabling truly automatic unattended operation.

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GPC and SEC Columns

GPC/SEC columns for all aqueous and organic applications

A wide range of columns for analytical GPC/SEC of synthetic and natural polymers and proteins.

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GPC and SEC Standards

A range of molecular weight standards for different applications of GPC/SEC

Malvern Panalytical offers a wide range of molecular weight standards for GPC/SEC for conventional and universal calibration as well as light scattering.

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The Malvern Panalytical OMNISEC can be used in many industry applications, including: