Using Taylor Dispersion Analysis for early stability assessment via a single kD measurement

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00:00:00 Welcome
00:00:13 Introduction
00:01:01 Using Taylor Dispersion Analysis for early stability assessment via a single kD measurement
00:01:20 Overview
00:01:52 Overview
00:01:56 Protein Stability and Aggregation
00:02:56 Protein Stability and Aggregation
00:03:41 Protein Stability and Aggregation
00:04:37 Measuring Protein Stability
00:06:06 Measuring Protein Stability
00:07:27 Characterisation across the pipeline
00:09:22 Measuring Protein Stability
00:10:02 What is kD and B22?
00:11:51 What is kD and B22?
00:13:00 Understanding kD and B22: Attractive and Repulsive Interactions
00:14:18 Understanding kD and B22: Attractive and Repulsive Interactions
00:15:01 Viscosity as an early predictor
00:15:41 Viscosity as an early predictor
00:16:12 Overview
00:16:32 Taylor Dispersion Analysis
00:17:35 Taylor Dispersion Analysis
00:18:13 Taylor Dispersion Analysis – Measuring Diffusion Coefficient
00:19:21 Taylor Dispersion Analysis – Measuring Hydrodynamic Radius
00:21:11 Taylor Dispersion Analysis – Frontal Injection
00:22:03 Taylor Dispersion Analysis – Measuring kD
00:23:29 Overview
00:23:42 Challenges of Peptide Characterisation
00:24:42 Selected Peptides
00:25:44 Peptide Characterisation
00:26:59 Comparison of the three Peptides based on kD
00:27:59 Formulation study of Bradykinin
00:28:56 Literature example: Peptide Characterisation using TDA
00:29:32 Literature example: Early developability screen of mAbs
00:31:14 Literature example: Early developability screen of mAbs
00:32:14 Conclusions
00:33:08 Thank you for your attentionAny questions?
00:37:29 Contact Information
The self-association characteristics of molecules in dilute solutions can provide an assessment of stability at an early stage in development. The diffusion interaction parameter (kD) is a measure of the propensity for self-association, but the determination of this parameter using existing methodologies requires several measurements over a concentration series. Here, we show how Taylor Dispersion Analysis can be used to generate a concentration gradient from which the kD can be extracted in a single, low volume measurement, making this an excellent choice of technology for early stability assessment, particularly for biopharmaceuticals.

Докладчики

Markos Trikeriotis studied Chemistry at the University of Crete in Greece, where he also completed his PhD in Biochemistry investigating the use of inorganic materials in drug delivery applications. He then moved to Cornell University, USA as a post-doctoral associate where he studied the applications of metal oxide nanoparticles in lithographic nano-patterning. Markos worked for a biotechnology start-up in Cardiff, UK and then joined the Microviscometry team at Malvern Instruments as an Applications Scientist.