Zetasizer Pro

Overview

The new Zetasizer Pro is your go-to instrument to measure a wide range of sample types and concentrations. It offers wide dynamic concentration capability and high sensitivity due to the Non-Invasive Back Scatter (NIBS) optical design. Measurement of particle size, molecular size, electrophoretic mobility, zeta potential and molecular weight can all be performed with ease.

The Adaptive Correlation feature enhances sample throughput by reducing measurement duration and providing greater sample knowledge. The deep learning-based data quality system gives you the confidence to know your data is the best it can be.

The optical filter wheel allows measurement of fluorescent samples without impacting overall instrument sensitivity.

Available in Blue Label and Red Label variants:

Choose Blue Label for routine sample investigation such as metallic colloids, metal oxides and pigments
Choose Red Label for more challenging sample types, such as proteins, surfactant solutions and low solid content samples

Works with OmniTrust: Malvern Panalytical's compliance solution for the regulated environment

Dynamic Light Scattering (DLS), which measures particle and molecule size, from 0.3 nm to 10 µm
M3-PALS with Constant Current Mode, for zeta potential and electrophoretic mobility measurements
Electrophoretic Light Scattering (ELS), which measures the zeta potential of particles and molecules, indicating sample stability and/or propensity to aggregate
Non-Invasive Back Scatter (NIBS), optics for versatile, high sensitivity, particle and molecular size measurements and molecular weight measurements
Adaptive Correlation, for faster, more reproducible sizing measurements with less sample preparation
Deep learning-based data quality system, for assessment of any size data quality issues, with clear advice on how to improve results
Optical filter wheel with fluorescence filter, to allow measurement of fluorescent samples without impairing overall system sensitivity.  Also includes polarization filters for DDLS measurements
A range of disposable and reusable cells are available to optimize the measurement in terms of sample volume and concentration
On-site upgradeability to Zetasizer Ultra, should your requirements change
The optional MPT-3 Autotitrator enables study of the effect of changes in pH

Which Zetasizer is right for me?

	Zetasizer Ultra	Zetasizer Pro	Zetasizer Lab
Size Measurement Principle	Non-Invasive Back Scatter (NIBS) Dynamic Light Scattering Dynamic Light Scattering (90° and 13°) Multi-Angle Dynamic Light Scattering (MADLS^®)	Non-Invasive Back Scatter (NIBS) Dynamic Light Scattering	Classical 90° Dynamic Light Scattering
Zeta Measurement Principle	ELS with M3-PALS and Constant Current Zeta Mode	ELS with M3-PALS and Constant Current Zeta Mode	ELS with M3-PALS and Constant Current Zeta Mode
Molecular Weight and B22	Static Light Scattering (90° and 173°)	Static Light Scattering (173°)	Static Light Scattering (90°)
Optical Filter Wheel (Fluorescence/DDLS)	Yes	Yes	No
Low Volume Disposable Sizing Cell with Extended Size Analysis Support	Yes	No	Yes
MADLS® (High Resolution DLS)	Yes	No	No
MADLS® - Particle Concentration/Titer	Yes²	No	No
21 CFR Part 11 compliant software	Optional via Omnitrust	Optional via Omnitrust	Optional via Omnitrust
Size
Range²	0.3 nm to 10 μm	0.3 nm to 10 μm	0.3 nm to 10 μm
Minimum Volume	3 μL	12 μL	3 μL
Zeta potential
Size Range	3.8 nm to 100 μm	3.8 nm to 100 μm	3.8 nm to 100 μm
Minimum Volume³	20 μL	20 μL	20 μL

¹ Red Label only
² 10 um by default. Option to extend for Zetasizer Lab and Zetasizer Ultra coming soon
³ Patented Diffusion Barrier method in capillary cell

Industry applications

The Zetasizer Advance Range is used in many industries, including:

Academia: The Zetasizer Lab is an essential analytical tool for many academic laboratories worldwide, finding use in all applications where particle or molecular size and zeta potential analysis is required. The breadth of applications covered by Zetasizer systems, coupled with tens of thousands of scientific citations, have earned these instruments a central space in many scientific faculties.

Bioscience: Variations in molecule concentration, external temperature, level of agitation and time all impact the stability of biological molecules. Instabilities mainly result in denaturation and aggregation of the molecule through which they can lose their biological function , elicit an immune response and consequently limit its therapeutic viability. Factors affecting therapeutic efficacy and immunogenicity therefore need to be monitored throughout the development pipeline especially at early stages to de-risk the biological drug molecule and limit late-stage failures. The Zetasizer range provides high quality, stability indicating data for biological drug molecules in a short time frame, enabling this measurement to be implemented throughout the development pipeline.

Food and drink: Zetasizer systems are used to analyze particle size and zeta potential to improve the appearance and taste of foods, drinks and flavorings, and optimize dispersion and emulsion stability to extend product shelf life and improve product performance.

Nanomaterials: Zetasizer measurements of nanoparticle size distribution, dispersion properties, stability and propensity to aggregate are crucial to the design of new nanomaterials. The very large surface area of such materials may result in novel physical and chemical properties, such as increased catalytic activity, improved solubility or unexpected optical or toxicological behavior.

Paints, inks and coatings: Paint, ink and coating formulations must be stable to keep them in an optimum state over time without change or aggregation. Particle size and zeta potential, measured by the Zetasizer instruments, play important roles in determining a product’s properties, such as dispersibility, color, strength, finish, durability and shelf life.

Pharmaceuticals: The correct particle size and zeta potential can help ensure the production of effective therapeutics, delivered safely. Zetasizer systems are used to characterize the stability and quality of dispersions, emulsions and creams, reducing formulation time and speeding new products to market.

Key applications

The Zetasizer Advance Range is suitable for a wide range of applications, including:

Colloidal Silica: Sizing of colloidal silica is a critical requirement. This is easily and reproducibly measured using the Zetasizer Lab Blue Label.

Titanium Dioxide: Titanium dioxide use is wide-ranging. When used in paints or inks, agglomerate or aggregate formation can diminish product performance. The Zetasizer Pro Blue Label, used alongside the MPT‑3 Autotitrator, characterizes the impact of salt concentration on the stability of TiO₂ and identifies the most stable pH region.

Ceramics: The strength of ceramics is linked to particle size, and the Zetasizer Ultra Blue Label is ideally placed to measure this. Al₂O₃ samples are often made of differently-sized particles which can easily be identified using MADLS. In this 0.1% w/v sample, the two particle size populations would not be distinguishable with a single angle DLS measurement.

Proteins: For protein researchers, early access to characterization data can be a challenge, due to low sample volumes. This is easily overcome using the Zetasizer Lab Red Label with the capillary cell to confirm that samples meet quality requirements and that production and purification processes are optimized.

Carbon Dots (C-dots): Photoluminescent C-dots are considered safer alternatives to quantum dots. Measuring fluorescent samples by DLS can be challenging due to additional photons emitted by the fluorophore. Available with NIBS on Zetasizer Pro Red Label, a filter removes this interference to provide accurate and reliable data.

Adeno-Associated Virus (AAV): Monitoring viral concentration is important during development and production optimization. By using the Multi-Angle DLS (MADLS) based particle concentration measurement in the Zetasizer Ultra Red Label, both the size distribution and titer of the virus can be measured within a few minutes.

The Zetasizer is one of the most user-friendly pieces of equipment I've used so far in my career. The instrument itself is easy to set up and operate. It has demonstrated high sensitivity for size measurements, and the datasets I've collected are consistent and reproducible."

Victoria Makal — University of Florida College of Medicine

Measurement principle	Non-Invasive Back Scatter (NIBS) Dynamic Light Scattering
Measurement angle	173°, 13°
Measurement range	0.3 nm - 10 μm
Minimum sample volume	12 μL
Concentration range	Minimum sample concentration: Blue Label: 0.2 mg/mL 15 kDa Protein Red Label: 0.1 mg/mL 15 kDa Protein Maximum sample concentration: ⁽³⁾ 40% w/v

Measurement principle	Mixed-Mode Measurement phase analysis light scattering (M3-PALS)
Size range	3.8 nm – 100 μm
Minimum sample volume	20 μL
Concentration range	Red Label: 1 mg/mL ⁽⁵⁾ to 40% w/v ⁽⁶⁾ Blue Label: 10 mg/mL ⁽⁵⁾ to 40% w/v ⁽⁶⁾
Sample conductivity range	Maximum: 260 mS/cm

Measurement principle	Static Light Scattering with Debye Plot
Molecular weight range	300 Da - 20 MDa
Accuracy	+/- 10%

Detector	Avalanche Photodiode
Laser safety	He-Ne (633 nm) Blue label: 4 mW Max Red label: 10 mW Max
Temperature control range	0 °C - 120 °C ⁷
Dimensions (W, D, H)	322 mm x 565 mm x 245 mm
Weight	19 kg
Power	AC 100-240 V, 50-60 Hz, 4.0 A
Power consumption	Max 100 W, Typical 45 W
Ambient conditions	+10°C to +35°C, 35 to 80% RH (Non-condensing)
Interface	USB 2.0 or higher
International standards	International Standards Organization ISO22412:2025 ISO13099-2:2025 US Pharmacopoeia (USP) 430 – Particle Size Analysis by Dynamic Light Scattering 432 – Determination of Zeta Potential by Electrophoretic Light Scattering 729 – Globule Size Distribution in Lipid Injectable Emulsions 1153 – Drug Products Containing Nanomaterials 1154 – Liposome Drug Products 1430 – Analytical Methodologies Based on Scattering Phenomena – General ASTM D4001 E2490 E2865 E3247

Notes	1 - Water as dispersant 2 - Indicative data available for particle sizes greater than 10 μm when using Extended Size Range analysis and low volume disposable sizing cell accessory 3 - Sample dependent 4 - Using patented diffusion barrier technique 5 - Measured using 15 kDa protein 6 - Measured using bile acid 7 - Dry nitrogen cell purge required for temperatures below dewpoint 8 - Accuracy 0.1°C at 25°C, 0.2°C at 0°C, 0.5°C at 90°C

Overview

The optical filter wheel allows measurement of fluorescent samples without impacting overall instrument sensitivity.

Available in Blue Label and Red Label variants:

Choose Blue Label for routine sample investigation such as metallic colloids, metal oxides and pigments
Choose Red Label for more challenging sample types, such as proteins, surfactant solutions and low solid content samples

Works with OmniTrust: Malvern Panalytical's compliance solution for the regulated environment

Dynamic Light Scattering (DLS), which measures particle and molecule size, from 0.3 nm to 10 µm
M3-PALS with Constant Current Mode, for zeta potential and electrophoretic mobility measurements
Electrophoretic Light Scattering (ELS), which measures the zeta potential of particles and molecules, indicating sample stability and/or propensity to aggregate
Non-Invasive Back Scatter (NIBS), optics for versatile, high sensitivity, particle and molecular size measurements and molecular weight measurements
Adaptive Correlation, for faster, more reproducible sizing measurements with less sample preparation
Deep learning-based data quality system, for assessment of any size data quality issues, with clear advice on how to improve results
Optical filter wheel with fluorescence filter, to allow measurement of fluorescent samples without impairing overall system sensitivity.  Also includes polarization filters for DDLS measurements
A range of disposable and reusable cells are available to optimize the measurement in terms of sample volume and concentration
On-site upgradeability to Zetasizer Ultra, should your requirements change
The optional MPT-3 Autotitrator enables study of the effect of changes in pH

Which Zetasizer is right for me?

	Zetasizer Ultra	Zetasizer Pro	Zetasizer Lab
Size Measurement Principle	Non-Invasive Back Scatter (NIBS) Dynamic Light Scattering Dynamic Light Scattering (90° and 13°) Multi-Angle Dynamic Light Scattering (MADLS^®)	Non-Invasive Back Scatter (NIBS) Dynamic Light Scattering	Classical 90° Dynamic Light Scattering
Zeta Measurement Principle	ELS with M3-PALS and Constant Current Zeta Mode	ELS with M3-PALS and Constant Current Zeta Mode	ELS with M3-PALS and Constant Current Zeta Mode
Molecular Weight and B22	Static Light Scattering (90° and 173°)	Static Light Scattering (173°)	Static Light Scattering (90°)
Optical Filter Wheel (Fluorescence/DDLS)	Yes	Yes	No
Low Volume Disposable Sizing Cell with Extended Size Analysis Support	Yes	No	Yes
MADLS® (High Resolution DLS)	Yes	No	No
MADLS® - Particle Concentration/Titer	Yes²	No	No
21 CFR Part 11 compliant software	Optional via Omnitrust	Optional via Omnitrust	Optional via Omnitrust
Size
Range²	0.3 nm to 10 μm	0.3 nm to 10 μm	0.3 nm to 10 μm
Minimum Volume	3 μL	12 μL	3 μL
Zeta potential
Size Range	3.8 nm to 100 μm	3.8 nm to 100 μm	3.8 nm to 100 μm
Minimum Volume³	20 μL	20 μL	20 μL

¹ Red Label only
² 10 um by default. Option to extend for Zetasizer Lab and Zetasizer Ultra coming soon
³ Patented Diffusion Barrier method in capillary cell

Industry applications

The Zetasizer Advance Range is used in many industries, including:

Academia: The Zetasizer Lab is an essential analytical tool for many academic laboratories worldwide, finding use in all applications where particle or molecular size and zeta potential analysis is required. The breadth of applications covered by Zetasizer systems, coupled with tens of thousands of scientific citations, have earned these instruments a central space in many scientific faculties.

Bioscience: Variations in molecule concentration, external temperature, level of agitation and time all impact the stability of biological molecules. Instabilities mainly result in denaturation and aggregation of the molecule through which they can lose their biological function , elicit an immune response and consequently limit its therapeutic viability. Factors affecting therapeutic efficacy and immunogenicity therefore need to be monitored throughout the development pipeline especially at early stages to de-risk the biological drug molecule and limit late-stage failures. The Zetasizer range provides high quality, stability indicating data for biological drug molecules in a short time frame, enabling this measurement to be implemented throughout the development pipeline.

Food and drink: Zetasizer systems are used to analyze particle size and zeta potential to improve the appearance and taste of foods, drinks and flavorings, and optimize dispersion and emulsion stability to extend product shelf life and improve product performance.

Nanomaterials: Zetasizer measurements of nanoparticle size distribution, dispersion properties, stability and propensity to aggregate are crucial to the design of new nanomaterials. The very large surface area of such materials may result in novel physical and chemical properties, such as increased catalytic activity, improved solubility or unexpected optical or toxicological behavior.

Paints, inks and coatings: Paint, ink and coating formulations must be stable to keep them in an optimum state over time without change or aggregation. Particle size and zeta potential, measured by the Zetasizer instruments, play important roles in determining a product’s properties, such as dispersibility, color, strength, finish, durability and shelf life.

Pharmaceuticals: The correct particle size and zeta potential can help ensure the production of effective therapeutics, delivered safely. Zetasizer systems are used to characterize the stability and quality of dispersions, emulsions and creams, reducing formulation time and speeding new products to market.

Key applications

The Zetasizer Advance Range is suitable for a wide range of applications, including:

Colloidal Silica: Sizing of colloidal silica is a critical requirement. This is easily and reproducibly measured using the Zetasizer Lab Blue Label.

Titanium Dioxide: Titanium dioxide use is wide-ranging. When used in paints or inks, agglomerate or aggregate formation can diminish product performance. The Zetasizer Pro Blue Label, used alongside the MPT‑3 Autotitrator, characterizes the impact of salt concentration on the stability of TiO₂ and identifies the most stable pH region.

Ceramics: The strength of ceramics is linked to particle size, and the Zetasizer Ultra Blue Label is ideally placed to measure this. Al₂O₃ samples are often made of differently-sized particles which can easily be identified using MADLS. In this 0.1% w/v sample, the two particle size populations would not be distinguishable with a single angle DLS measurement.

Proteins: For protein researchers, early access to characterization data can be a challenge, due to low sample volumes. This is easily overcome using the Zetasizer Lab Red Label with the capillary cell to confirm that samples meet quality requirements and that production and purification processes are optimized.

Carbon Dots (C-dots): Photoluminescent C-dots are considered safer alternatives to quantum dots. Measuring fluorescent samples by DLS can be challenging due to additional photons emitted by the fluorophore. Available with NIBS on Zetasizer Pro Red Label, a filter removes this interference to provide accurate and reliable data.

Adeno-Associated Virus (AAV): Monitoring viral concentration is important during development and production optimization. By using the Multi-Angle DLS (MADLS) based particle concentration measurement in the Zetasizer Ultra Red Label, both the size distribution and titer of the virus can be measured within a few minutes.

The Zetasizer is one of the most user-friendly pieces of equipment I've used so far in my career. The instrument itself is easy to set up and operate. It has demonstrated high sensitivity for size measurements, and the datasets I've collected are consistent and reproducible."

Victoria Makal — University of Florida College of Medicine

High performance, versatile and robust

Overview

Features

Which Zetasizer is right for me?

Industry applications

Key applications

Specification

Accessories

System enhancements

Cell and cuvette options

Smart Manager

Smart Install

User manuals

Software downloads

Unleash the full potential of your Malvern Panalytical solution

Unmatched uptime and performance

Advanced analysis and actionable insights

Easy start and streamlined routine operations

Want to speak to a service expert or find the services that are just right for you?

Publications

Overview

Features

Which Zetasizer is right for me?

Industry applications

Key applications

Specification

Accessories

System enhancements

Cell and cuvette options

Smart Manager

Smart Install

User manuals

Software downloads

Unleash the full potential of your Malvern Panalytical solution

Unmatched uptime and performance

Advanced analysis and actionable insights

Easy start and streamlined routine operations

Want to speak to a service expert or find the services that are just right for you?

Publications

Advanced light scattering with NIBS for exceptional size measurement.